Sampling procedure for yeast culture (SOP ID: 0012)

Revision nr.:
Release date:
November 19, 2019

Last release date:

February 3, 2020
Ada Serena Marletta


Ada Serena Marletta

1. Scope

This Standard Operating Procedure provides a step-by-step protocol for sampling yeast culture during its growth in a bench bioreactor. The sampling procedure mainly depends upon the method employed to grow yeast, and sampling volumes may vary depending on the type of measurement to be performed downstream. Drafting of this Procedure is in progress.


2. Background

This Standard Operating Procedure is intended for qualified personnel who was trained according to the ISO-9001 quality framework.

3. Prerequisites

Documents required:

  • MIAMI quality manual
  • SOP ID 0001

Material required:

  • Generic lab-, glass- and plastic-ware
  • MicroClave Connectors
  • Syringe w/Spinning Spiros (3 ml, 5ml, 10 ml)
  • Laminar Flow Hood
  • Ethanol
  • Spectrophotometer
  • Plastic cuvettes

4. Changes since last revision

No changes were made since last version

5. Role and responsibilities

Author (Au): Staff scientist properly trained to draft, review and disseminate standard operating procedures within the framework of ISO-9001

Approver (Ap): PI with the authority to approve, reject and withdraw standard operating procedures

MIAMI Quality Manager (QM): MIAMI authorized Quality Manager

Operator (Op): Staff scientist properly trained to execute the standard operating procedure

6. Monitoring Requirements

The present document can be reviewed at any time required by the author and the MIAMI Quality Manager.
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