Yeast growth protocol in a bench bioreactor (SOP ID: 0010)

Revision nr.:
Release date:
November 19, 2019

Last release date:

January 28, 2020
Ada Serena Marletta


Ada Serena Marletta

1. Scope

This Standard Operating Procedure provides a step-by-step protocol for growing yeast cells into a 200 ml bench bioreactor. Drafting of this Procedure is in Progress.

2. Background

This Standard Operating Procedure is intended for qualified personnel who was trained according to the ISO-9001 quality framework.

3. Prerequisites

Documents required:

  • MIAMI quality manual
  • SOP ID 0001

Material required:

  • Generic lab-, glass- and plastic-ware
  • yeast glycerol stocks
  • isotherm rack
  • orbital shaker
  • Bioreactor Solaris IO 200
  • Hamilton pH probe
  • SC medium
  • SC agar plates
  • MicroClave Connectors
  • Himalia sterile syringe 3 mL with non-return valve (10 ml, 5 ml, 3 ml)
  • Duran® Connection Cap System GL25 with PBT cap ports, 2, stainless steel ports, PTFE insert
  • Duran Bottles
  • Tubing clamps, Squeeze-Fix
  • Eutectic plates
  • 0.22 μm autoclavable filters
  • Bunsen burner
  • Silicone pipes
  • Spectrophotometer
  • Cuvettes
  • Polypropylene glycol P 2000
  • pH standard solutions
  • Ethanol

4. Changes since last revision

No changes were made since last version

5. Role and responsibilities

Author (Au): Staff scientist properly trained to draft, review and disseminate standard operating procedures within the framework of ISO-9001

Approver (Ap): PI with the authority to approve, reject and withdraw standard operating procedures

MIAMI Quality Manager (QM): MIAMI authorized Quality Manager

Operator (Op): Staff scientist properly trained to execute the standard operating procedure

6. Monitoring Requirements

The present document can be reviewed at any time required by the author and the MIAMI Quality Manager.
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