Priming and loading the Flow Cell for nanopore sequencing (SOP ID:0006)

Revision nr.:
Release date:
November 26, 2019

Last release date:

November 26, 2019
Ada Serena Marletta


Ada Serena Marletta

1. Scope

This Standard Operating Procedure provides a step-by-step protocol for priming the flow cell and loading the cDNA library into it before running nanopore sequencing.

2. Background

This Standard Operating Procedure is intended for qualified personnel who was trained according to the ISO-9001 quality framework.

3. Prerequisites

Documents required:

  • MIAMI quality manual
  • SOP ID 0001

Material required:

  • Generic lab-, glass- and plastic-ware
  • MinION Mk1B (MIN-101B)
  • Flow Cell (FLO-MIN106D)
  • Direct cDNA Sequencing Kit (SQK-DCS109)
  • Flow Cell Priming Kit (EXP-FLP002)
  • 1.5 ml Eppendorf DNA LoBind tubes
  • Nuclease-free water (e.g. ThermoFisher, cat #AM9937)

4. Changes since last revision

No changes were made since last version

5. Role and responsibilities

Author (Au): Staff scientist properly trained to draft, review and disseminate standard operating procedures within the framework of ISO-9001

Approver (Ap): PI with the authority to approve, reject and withdraw standard operating procedures

MIAMI Quality Manager (QM): MIAMI authorized Quality Manager

Operator (Op): Staff scientist properly trained to execute the standard operating procedure

6. Monitoring Requirements

The present document can be reviewed at any time required by the author and the MIAMI Quality Manager
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