Plasmid DNA sequencing (SOP ID: 0002)
Last release date:
This Standard Operating Proceducere provides a step-by-step protocol for DNA plasmid sequencing with pre-mixed sequencing primers.
- 1,5 ml safe-lock tubes
- 0,2 ml strip tubes for PCR
- 96-wells plates for PCR
- Sequencing Primers
- Sequencing labels (if required by DNA sequencing provider)
- Biology grade water
4. Changes since last revision
5. Role and responsibilities
Author (Au): Staff scientist properly trained to draft, review and disseminate standard operating procedures within the framework of ISO-9001
Approver (Ap): PI with the authority to approve, reject and withdraw standard operating procedures
MIAMI Quality Manager (QM): MIAMI authorized Quality Manager
Operator (Op): Staff scientist properly trained to execute the standard operating procedure
Other (Oth): Third-party personnel and/or institution and/or authority, and/or service provider.